The author of the book: R. Since publication of the previous edition of this benchmark text, numerous groundbreaking advances have occurred in stem cell research, cloning, tissue engineering, and in vitro toxicity testing. These and other developments have been incorporated into this fully revised and expanded Fifth Edition of "Culture of Animal Cells". In addition, to answer the needs of the exponential increase in newcomers to cell culture, particularly in the biopharmaceutical industry, a completely new chapter on training in cell culture technology has been introduced. The most complete resource PDF on the techniques, equipment, principles, and practices of animal cell culture, this text offers a complete background related to growth of animal cells in culture. Beginning with laboratory design, safety, validation and bioethics, then continuing with preparation of media, primary culture and cell lines, through to characterization and authentication, contamination, specialized techniques, and troubleshooting, the coverage includes: an all-new section of training exercises, separated into basic, intermediate, and advanced procedures, cross-referenced to the relevant protocols New coverage of stem cells, bioethics, validation, cloning, cell signaling, in vitro toxicity testing, and tissue engineering; an expanded full-color atlas ePub section, with images of primary culture, cell lines, subculture, differentiation, cancer cells and transformation, three-dimensional culture, contamination, and specialized equipment; enhanced treatment of troubleshooting, with full cross-referencing to the relevant protocols and sections of text; and fully updated references.
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Ian Freshney This is the most complete resource on the techniques, equipment, principles, and practices of animal cell culture. Since publication of the previous edition of this benchmark text, numerous types of equipment and materials have changed and new specialized techniques have been developed, particularly in the area of stem cell research and tissue engineering.
These and other developments have been incorporated into this rigorously revised and expanded sixth edition of Culture of Animal Cells, the leading text in the basic methodology of cell culture. This authoritative volume offers a complete background related to growth of animal cells in culture. It contains many specialized protocols and advice on how to apply some of the basic protocols to teaching cell culture. Ian Freshney, PhD. Author R. He is a world-renowned expert on cell culture technique and has authored and edited numerous successful books, including the hugely popular Culture of Animal Cells now in its 6th Edition , and Culture of Human Stem Cells.
Culture of Animal Cells: A Manual of Basic Technique and Specialized Application, 6th Ed has been very well received by the scientific community, as have many of your other titles.
What do you think sets your books apart and makes them so successful compared to other titles in the field? I would say there are three major components: 1 The books are designed as practical guides with detailed single-step instructions which should be sufficient without further recourse to the literature. I struggled with the need to update but at the same time preserve basic procedures which have not changed markedly from the previous edition.
This, of course, led to the dilemma of deciding what to retain and what to leave out. I also thought long and hard about what specialized protocols to add without compromising the basic technique role of the book. What do you find rewarding about authoring and editing books in the sciences? It forces me to keep up-to-date with developments in cell culture, and in turn helps me to be a better teacher of cell culture.
I also get satisfaction from converting what are sometimes complex instructions into one simple straightforward procedure. What inspired you to write your first book? I was invited to prepare a textbook on cell culture for a college course.
What advice would you offer to scientists writing or editing their first book? Even if it is to be professionally indexed you will find terms that the indexer does not. How would you compare the experiences of writing a book and writing a journal article? Both require a clear concise style, but the author has more freedom to express ideas and opinions when writing a book. Do you find that the role of books in the scientific research community has changed over the years? Are they valued more or less today than they were years ago?
Rapid changes in technology mean that some books will become outdated without regular updates, which are not always feasible in printed copy. Yet, while there is an increasing tendency to provide instructions and protocols online, many people will still find reassurance from using a textbook with an established reputation.
How do you envision the evolution of science writing over the next years? Certain journals will still tend to be regarded as more reliable than others, as indicated by their citation indices.
However, the number of free public access journals will probably increase, presumably with payment to submit articles. There will be a continuing need for editorial control of journal content due to the proliferation of unedited, non-reviewed material appearing online. What do you feel has been the most significant scientific discovery that has been made during your lifetime? I can really only speak for biology where the elaboration of the genetic code is probably the most significant development.
The ability to regulate gene expression and the resultant plasticity of the cell phenotype will create major opportunities in cell culture and its relevance to tissue in vivo. What are you reading right now? This questionnaire! Otherwise, mostly novels and current journals for which I have alerts.
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Books by R. Ian Freshney
Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications